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Fig. 6 | BMC Immunology

Fig. 6

From: Mesenchymal stem cell conditioned medium alleviates oxidative stress injury induced by hydrogen peroxide via regulating miR143 and its target protein in hepatocytes

Fig. 6

miRNA143 target proteins relative content examined by western blot analysis. a-b. HK2 and ADRB1 level decreased after H2O2 injury, and increased after MSC-CM treatment (Control group = 1, * P < 0.05 vs. control group; # P < 0.05 vs. H2O2 group). c. The expression of miR143 in transfected group. d and f. The expression of HK2 and ADRB1 in miRNA transfected 60 h group(NC: miRNA negative control, NC = 1, * P < 0.05 vs. Inhibitor NC group; # P < 0.05 vs. NC group; ^ denotes P < 0.05: Inhibitor group vs. Mimics group or Inhibitor NC group vs. NC group). e and g. The expression of HK2 and ADRB1 in the siRNA group under H2O2 injury (H2O2 group: L02 incubated with 0.8 mM H2O2 for 2 h, siRNA + H2O2 group: H2O2 treatment following siRNA transfected 60 h; H2O2 group = 1, * P < 0.05 vs. control group; # P < 0.05 vs. H2O2 group; α P < 0.05 vs. NC+ H2O2 group; β P < 0.05 vs. Inhibitor NC+ H2O2 group; ^ denotes P < 0.05: Inhibitor + H2O2 group vs. Mimics + H2O2 group or Inhibitor NC+ H2O2 group vs. NC+ H2O2 group). All values were expressed as mean ± SD of ten independent experiments

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BMC Immunology

ISSN: 1471-2172

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