Fig. 9

a-b Detection of donor-specific antibodies from rats after syngeneic or allogeneic kidney transplantation treated with daily CyA for 6 or 28 days, or CyA on alternating days until d28. Donor splenocytes were incubated with recipient serum and then stained with (a) anti-rat IgM-antibody or (b) anti-rat IgG-antibody in duplicates and measured by flow cytometry. Percentages of IgG or IgM-positive cells are shown. Data is shown as single data points of each group on a scatter plot. Groups consisted of 5–6 animals. Statistical analysis is shown (Mann-Whitney U Test). Significance is shown as * p < 0.05 compared to “SynTxd6”, # p < 0.05 compared to “Txd28CyAalt”