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Fig. 3 | BMC Immunology

Fig. 3

From: Beta-2 adrenergic receptors increase TREG cell suppression in an OVA-induced allergic asthma mouse model when mice are moderate aerobically exercised

Fig. 3

TREG cells lacking β2-AR expression exhibit decreased intracellular cAMP levels and TREG cell suppressive function. TREG cells (CD4+CD25+) with (WT TREG) or without (β2-AR−/− TREG) β2-adrenergic receptors were negatively isolated and; (a) assessed for cyclic-AMP levels after lysing using RIA. (n = 5 in triplicate) *P ≤ 0.05 between groups where indicated. b co-cultured with wildtype CD4+CD25 naive Th cells using artificial Th cell activation (anti-CD3 and anti-CD28) for 72 h. Th cell proliferation was assessed using 3H–thymidine incorporation. *P < 0.01 for repeated measures ANOVA analysis. All data are shown as mean +/− SEM of triplicates for three independent experiments

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