Fig. 4From: Beta-2 adrenergic receptors increase TREG cell suppression in an OVA-induced allergic asthma mouse model when mice are moderate aerobically exercisedTreatment with formoterol is sufficient to increase TREG suppressive function. TREG cells isolated from all experimental mouse groups (S, E, SO, EO) were treated with a β2-adrenergic receptor agonist (formoterol) for 30 min. Formoterol-treated TREG cells were co-cultured with untreated non-sensitized naive Th cells using artificial Th cell activation (anti-CD3 and anti-CD28) for 72 h. Th cell proliferation was assessed using 3H–thymidine incorporation. a non-sensitized TREG cells (S and E). (n = 7–10) *P < 0.0001 between groups where indicated. Two-way ANOVA: β2-agonist treatment - p < 0.0001, Exercise treatment - p = 0.05, Interaction - n.s. or (b) OVA-sensitized TREG cells (SO and EO). (n = 7–10) *P < 0.0002 between groups where indicated. Two-way ANOVA: β2-agonist treatment - p < 0.0001, Exercise treatment - p = 0.0031, Interaction – p = 0.005. All data are shown as mean +/− SEM of triplicates of seven independent experimentsBack to article page