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Fig. 4 | BMC Immunology

Fig. 4

From: Beta-2 adrenergic receptors increase TREG cell suppression in an OVA-induced allergic asthma mouse model when mice are moderate aerobically exercised

Fig. 4

Treatment with formoterol is sufficient to increase TREG suppressive function. TREG cells isolated from all experimental mouse groups (S, E, SO, EO) were treated with a β2-adrenergic receptor agonist (formoterol) for 30 min. Formoterol-treated TREG cells were co-cultured with untreated non-sensitized naive Th cells using artificial Th cell activation (anti-CD3 and anti-CD28) for 72 h. Th cell proliferation was assessed using 3H–thymidine incorporation. a non-sensitized TREG cells (S and E). (n = 7–10) *P < 0.0001 between groups where indicated. Two-way ANOVA: β2-agonist treatment - p < 0.0001, Exercise treatment - p = 0.05, Interaction - n.s. or (b) OVA-sensitized TREG cells (SO and EO). (n = 7–10) *P < 0.0002 between groups where indicated. Two-way ANOVA: β2-agonist treatment - p < 0.0001, Exercise treatment - p = 0.0031, Interaction – p = 0.005. All data are shown as mean +/− SEM of triplicates of seven independent experiments

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