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Fig. 6 | BMC Immunology

Fig. 6

From: Beta-2 adrenergic receptors increase TREG cell suppression in an OVA-induced allergic asthma mouse model when mice are moderate aerobically exercised

Fig. 6

β2-AR−/− TREG cells isolated from moderate aerobic exercised mice do not increase TREG suppressive function. Wildtype BALB/c mice were TREG cell depleted using anti-CD25 intravenous 24 h prior to receiving 5 × 105 wildtype or β2-AR−/− TREG cells. Mice underwent OVA-sensitization and exercise protocols (see Fig. 1). At the end of the protocol, TREG cells from each group were co-cultured with non-sensitized naive Th cells using artificial Th cell activation (anti-CD3 and anti-CD28) for 72 h. Th cell proliferation was assessed using 3H–thymidine incorporation. (n = 8–14) *P ≤ 0.005 between groups where indicated. Two-way ANOVA: exercise treatment – p = 0.04, β2-AR treatment – n.s., Interaction – p = 0.04. All data are shown as mean +/− SEM of triplicates of two independent experiments

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