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Fig. 7 | BMC Immunology

Fig. 7

From: Beta-2 adrenergic receptors increase TREG cell suppression in an OVA-induced allergic asthma mouse model when mice are moderate aerobically exercised

Fig. 7

Th2 cytokines are not attenuated in moderately exercised mice after β2-AR−/− TREG cell adoptive transfer. Wildtype BALB/c mice were TREG cell depleted using anti-CD25 intravenous 24 h prior to receiving 5 × 105 wildtype or β2-AR−/− TREG cells. Mice underwent OVA-sensitization and exercise protocols (see Fig. 1). At the end of the protocol, bronchio-aveolar lavage fluid samples were collected from each mouse group and assessed for Th2 cytokine levels: (a) IL-4, Two-way ANOVA for IL-4: exercise treatment – P = 0.002, TREG treatment – P = 0.007., Interaction – P = 0.0008. b IL-5, Two-way ANOVA for IL-5: exercise treatment – P = 0.005, TREG treatment – P = 0.0004., Interaction – P = 0.016. c IL-13, Two-way ANOVA for IL-5: exercise treatment – n.s., TREG treatment – P = 0.0005., Interaction – P = 0.05. Th2 cytokine levels were assessed using a standard ELISA. (n = 6–8) All data are shown as mean +/− SEM of triplicates of two independent experiments. *P ≤ 0.05 between groups using a student t-test where indicated

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