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Fig. 2 | BMC Immunology

Fig. 2

From: Pathogen recognition by NK cells amplifies the pro-inflammatory cytokine production of monocyte-derived DC via IFN-γ

Fig. 2

Sensing of bacterial PAMPs triggers NK cell activation and cytokine secretion. NK cells (2.5 × 105 cells/well) were activated overnight in round-bottom 96-well plates with various bacterial PAMPs in the absence (white bars) or presence (gray bars) of IL-2 in serum-free medium. As a negative control, NK cells were incubated with medium alone. a After 16-18 h of incubation IFN-γ, TNF-α, CCL-3, and CCL5 were determined in the culture supernatant by CBA. b After 16-18 h of incubation cells were harvested and stained for CD69, CD25, and CD83. Expression of these markers was analysed by flow cytometry. All data are presented as mean + SEM and are representative of at least 3 independent experiments (3 ≤ n ≤ 12). Kruskal-Wallis test significance as compared to negative control with and without IL-2, respectively. * P≤0.05, ** P≤0.01, *** P≤0.001, **** P≤0.0001

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