Fig. 3
Soluble factors derived from viral and bacterial-triggered NK cells increase the pro-inflammatory DC cytokine profile. NK cells (2.5 × 105 cells/well) were activated overnight in round-bottom 96-well plates with various viral and bacterial PAMPs (indicated on x-axis) in the presence of IL-2. As control, viral and bacterial PAMPs were stored overnight in the incubator without the presence of NK cells. iDC were matured in these cell-free NK cell-derived supernatants (gray bars) or control supernatants (white bars) supplemented with IL-4 (500 U/ml) and GM-CSF (500 U/ml). After 48 h, DC-derived cytokines were determined in the culture supernatant by CBA. a IL-12p70 production. Mean + SEM of n ≥ 9 independent experiments is shown. b IL-1β production. Mean + SEM of n ≥ 8 independent experiments is shown. c IL-6 production. Mean + SEM of n ≥ 3 independent experiments is shown. Mann-Whitney U test comparing absence and presence of NK cell-derived supernatant (white vs. gray bars). * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001, **** P ≤ 0.0001