Fig. 6

NK cell-derived IFN-γ but not TNF-α is necessary to modulate the pro-inflammatory DC cytokine profile. a Supplementation of rhIFN-γ during DC maturation and its effect on cytokine and chemokine secretion is shown. iDC were matured in serum-free medium supplemented with FMKp, IL-4 and GM-CSF in the presence of increasing concentrations of rhIFN-γ. Cytokine and chemokine profiles were determined by CBA in the culture supernatants after 48 h of maturation. The individual donors are shown. b Blocking of IFN-γ and TNF-α during DC maturation and its effect on cytokine and chemokine secretion is shown. iDC were matured in the cell-free supernatant derived from FMKp-activated NK cells in the presence of IL-2. As indicated on the y-axis, various blocking antibodies were added: anti-IFN-γ, anti-IFNGR1, anti-TNF-α, anti-TNFR1, and anti-TNFR2. iDC matured with NK cell-derived supernatant in the absence of blocking antibodies (untreated) were used as reference value and set at 100% capacity to produce a cytokine/chemokine (x-axis). The negative control represents the capacity of DC to produce the indicated cytokine upon maturation with FMKp in absence of NK cell- derived supernanant. Mean + SEM of 3 independent experiments is shown. Statistical analyses were performed on normalized data using paired t-test comparing untreated DC versus DC conditions containing blocking antibodies. Significance is indicated by *