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Fig. 1 | BMC Immunology

Fig. 1

From: MCSF drives regulatory DC development in stromal co-cultures supporting hematopoiesis

Fig. 1

Production of cells in stromal co-cultures. Co-cultures were established by overlay of Lin bone marrow over 5G3 stroma. (a) Hematopoietic cells adhered to stroma were observed under phase contrast microscopy at day 21. Bar represents 100 μm. (b) Gating procedure to detect CD11b+CD11c+ cells is shown for cells produced in co-cultures at 28-days. (c) Cell production was assessed at 14, 21 and 28 days using antibody staining and multicolor flow cytometry. Production of the CD11bCD11c, CD11b+CD11c, CD11b+CD11c+, CD11b+CD11c+MHC-II(L-DC) and CD11b+CD11c+MHC-II+ (cDC-like) subsets was calculated in terms of proportion of each subset amongst the total CD11b+ and/or CD11c+ population. Graphs show mean ± S.E. for triplicate co-cultures. Cell production in terms of subset size is statistically different (p ≤ 0.05) for days 21 and 28 compared with day 14 in terms of increased dendritic cell and reduced myeloid cell production

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