Fig. 4From: MCSF drives regulatory DC development in stromal co-cultures supporting hematopoiesisCytokine release upon DC activation of antigen-specific T cells. The release of IL-2, IL-4, IL-10 and IFN-γ by OT-I and OT-II T cells following stimulation with different APC subsets was investigated. L-DC and cDC-like subsets were sorted from 21-day co-cultures established with Lin− BM from Act-mOVA mice above 5G3 stroma. Control CD8+ cDC and CD8− cDC subsets were sorted from Act-mOVA spleen. These four APC subsets, and a NIL control, were incubated with column purified CD8+ OT-I T cells and CD4+ OT-II T cells from spleen for 4 days. The supernatant of co-cultures was collected, tested for the presence of cytokines using an Elisa array kit (Qiagen), and production calculated as A450 of sample relative to A450 of a standard provided with the kitBack to article page