MCSF drives regulatory DC development in stromal co-cultures supporting hematopoiesis

BMC Immunology

Table 1 Genes upregulated in L-DC or cDC-like cells

Gene	L-DC/cDC-like	Gene	cDC-like/L-DC
Ccl20	7.30	Fcer1a	14.44
Il12a	5.53	Ccl8	7.96
Cd8a	5.38	Ccl7	5.78
Cxcl12	4.99	Ccr3	5.43
Ccl19	4.88	Fcgrt	5.25
Ifng	4.31	Fcer1g	5.21
Cxcl10	4.16	Inhba	4.83
Vcl	3.86	Cd2	3.92
Cd1d2	3.75	Cd36	3.79
Relb	3.50	Cxcl2	3.64
Il8ra	3.48	Tlr2	3.51
Adamdec1	3.41	Ccl2	3.42
Il2	3.31	Mif	3.42
Ifit3	3.29	Ccl12	3.39
Erbb2	3.11	Ccr5	3.37
Icam2	2.99	Csf1r	2.72
Ccl5	2.84	Ccr1	2.62
Ccr2	2.58	Cd33	2.55
Icam1	2.39	Itgb2	2.22
Ccl11	2.34	Il16	2.19
Fcer2a	2.33	Cebpa	2.18
Cd80	2.31	Tnf	2.16
Cd4	2.25	Cdc42	2.00
Cd40	2.23
Trap1	2.14
Il12b	2.11
Il6	2.11

Realtime PCR was performed using an RT² Profiler PCR Array (SABiosciences) to measure the relative expression of genes common to murine dendritic and antigen presenting cells by sorted populations of L-DC and cDC-like cells isolated from 28-day co-cultures. Genes upregulated ≥ 2-fold in L-DC or cDC-like cells are listed

ISSN: 1471-2172