Fig. 4

KCTD9 shRNA inhibited KCTD9 expression and ameliorates MHV-3 induced fulminant hepatitis in vivo. a ALT levels of infected mice delivered with distinct plasmid at 24 h,48 h and 108 h after MHV-3 infection. b Hematoxylin and eosin (H&E) staining of livers sections from mice 4 days post MHV-3 infection. Black arrow indicated the inflammatory cells area within black lines indicated liver necrosis. Magnification: 400 X. c Immunochemistry staining against mKCTD9 on liver sections of mice injected with various plasmid at 36 h after MHV-3 infection. Blank points to IgG stained section; The brown staining area indicates signals of KCTD9 protein. Magnification: 400 X. *p < 0.05, **p < 0.01, Means ± SEM of 5 to 7 independent experiments were represented