Table 1 Primers used in mKCTD9 cloning, shRNA and quantitative PCR
From: Interference with KCTD9 inhibits NK cell activation and ameliorates fulminant liver failure in mice
Gene | Primer |
|---|---|
Mouse KCTD9 (for cloning) | anti-sense 5’ ATAAGAATGCGGCCGCGCACCTCTGGGAACAAGC 3’ |
sense 5’ CCCAAGCTTGTGCGTGCGTGAGGAGTGT 3’ | |
mKCTD9-shRNA1 | anti-sense 5’ AAGCTTAAAAATTGACCGAAGTCCTGAGTATCTCTTGAATACTCAGGACTTCGGTCAAAAACAAGGCTTTTC 3’ |
mKCTD9-shRNA2 | anti-sense 5‘AAGCTTAAAAAGCCAGATGACAGGGATTAATCTCTTGAATTAATCCCTGTCATCTGGCAAACAAGGCTTTTC 3’ |
shRNA-Negative Control | anti-sense 5’ AAGCTTAAAAAGACGCAACGGAAGTGTTAATCTCTTGAATTAACACTTCCGTTGCGTCAAACAAGGCTTTTC 3’ |
mKCTD9-shRNA | sense 5’ GTAGGATCCATCCGACGCCGCCATCTC 3’ |
mKCTD9 (for qPCR) | anti-sense 5’ ACGATACTTCACAACAACAAGG 3’ |
sense 5’ CCTGGCTTCTTCTAGCACAC 3’ | |
GAPDH (for qPCR) | anti-sense 5’ CGGATTTGGTCGTATTGGG 3’ |
sense 5’ CTCGCTCCTGGAAGATGG 3’ |