Table 2 Primer sequences used for real time PCR quantifications
From: The expression analysis of Fra-1 gene and IL-11 protein in Iranian patients with ulcerative colitis
Gene | Primer sequences (5′-3′) | Tm (°C) | Amplicon Size (bp) |
|---|---|---|---|
fra-1 | F: -5́ TGACCACACCCTCCCTAACTC -3́ | 60.83 | 100 bp |
R: -5́ CTGCTGCTACTCTTGCGATGA -3́ | 60.47 | ||
GAPDH | F: -5́ ACAGTCAGCCGCATCTTC -3́ | 57.39 | 169 bp |
R: -5́ CTCCGACCTTCACCTTCC -3́ | 57.66 |