Fig. 1
Detection of IFN-γ production following BCG infection. Four groups of C57BL/6 mice (n = 6) s.c. vaccinated with 1 × 108 CFU BCG were sacrificed at different time points, and their spleens and inguinal LNs were removed. Increased IFN-γ levels were detected in the culture supernatants of splenocytes (a) and inguinal LN cells (b). Results are representative of three independent experiments and presented as means ± SEM. Statistical significance was determined using Student’s t-test (*P < 0.05, **P < 0.01, ***P < 0.001). CM, culture medium