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Fig. 3 | BMC Immunology

Fig. 3

From: Rapid loss of early antigen-presenting activity of lymph node dendritic cells against Ag85A protein following Mycobacterium bovis BCG infection

Fig. 3

Expression of MHC II, CIITA, and co-stimulatory molecules on DCs following BCG infection. Inguinal LNs were obtained at different time points following s.c. infection of mice with 1 × 108 CFU BCG (n = 6) and were sorted and stained with a panel of mAbs to detect cell-surface expression of CD40 (a), CD54 (b), CD80 (c), and CD86 (d) by FACS. Inguinal LNs were obtained from five groups of mice (n = 6) at different time points following s.c. injection of 1 × 108 CFU BCG. Transcription levels of MHC II, total CIITA (CIITA T), and CIITA type I (CIITA I) were analyzed using real-time PCR (e), and DCs were sorted and stained with mAbs to detect MHC II by FACS (f). The results are representative of three independent experiments and presented as means ± SEM. Statistical significance was determined using Student’s t-test (*P < 0.05)

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BMC Immunology

ISSN: 1471-2172

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