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Fig. 2 | BMC Immunology

Fig. 2

From: The differences in immunoadjuvant mechanisms of TLR3 and TLR4 agonists on the level of antigen-presenting cells during immunization with recombinant adenovirus vector

Fig. 2

The effect of TLR3 and TLR4 agonists on the efficacy of reactivation of H1-specific T cells. a-f balb/c mice were immunized (i.m.) with 108 PFU rAdTet-off H1. Forty days after immunization mice were euthanized, the pool of CD8+ (a-c) and CD4+ (d-f) T cells from the spleen of two immune mice was re-activated in vitro. Sorted CD8+ and CD4+ T cells were co-cultured with bone marrow derived DCs preloaded with 3.5 (a, d), 35 (b, e), or 350 (c, f) PFU/cell rAdTet-off H1 in the presence of 0–10 μg/ml agonists of TLR3 (Poly I:C) or TLR4 (LPS, IMM). The number of reactivated IFNγ-producing T-cells were detected by ELISPOT and calculated for 1 million spleen cells. Shown are M ± SD, statistically significant differences (p < 0.05) are indicated by asterisks

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BMC Immunology

ISSN: 1471-2172

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