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Fig. 3 | BMC Immunology

Fig. 3

From: The differences in immunoadjuvant mechanisms of TLR3 and TLR4 agonists on the level of antigen-presenting cells during immunization with recombinant adenovirus vector

Fig. 3

Efficacy of reactivation of antigen-specific T cells depending on the level of expression of the target rAd antigen in APCs. a-c the relative level of expression mRNA of H1 in DCs loaded with 3.5 (a), 35 (b), or 350 (c) PFU/cell rAdTet-off H1 in the presence of 0–10 μg/ml agonists of TLR3 (Poly I:C) or TLR4 (LPS, IMM). cDNA was obtained from total RNA extracts of DC and used as a template for quantitative PCR with primers specific for H1 and β-actin genes. The expression values of H1 gene were normalized with the expression of β-actin gene. d DCs were transduced with rAdTet-off H1 (100 PFU per cell) in the presence of 10 μg/ml agonists of TLR3 (Poly I:C) or TLR4 (LPS, IMM), 24 h after transfection cells were stained with primary (H1-specific) and secondary fluorochrome labeled antibodies, the percentage of H1-positive DCs in the test samples was detected by flow cytometry. Shown are M ± SD, statistically significant (p < 0.05) differences are indicated by asterisks. e dependence of H1-specific T cells reactivation efficiency and rAdTet-off H1 mRNA expression from the viral loading of DCs. f-h correlation of rAdTet-off H1 mRNA expression in DCs activated with TLR4 agonists – LPS (f), IMM (g), and TLR3 agonist Poly I:C (h) with an efficiency of reactivation of H1-specific T cells

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BMC Immunology

ISSN: 1471-2172

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