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Fig. 5 | BMC Immunology

Fig. 5

From: The differences in immunoadjuvant mechanisms of TLR3 and TLR4 agonists on the level of antigen-presenting cells during immunization with recombinant adenovirus vector

Fig. 5

Paracrine transfer of enhancement and suppression of rAd expression in APCs. a Scheme of the experiment: peritoneal macrophages were incubated for 3 h in the presence of 10 μg/ml agonists of TLR3 (Poly I:C) and TLR4 (LPS, IMM) or without activators, cells were washed three times from the activators and mixed with non-activated Celltrace ™ Violet-labeled cells. Mixed populations of cells were transfected with rAd-GFP (100 PFU/cell), as a negative control - cells without transduction (no Ad). After 24 h, a mean fluorescence intensity of GFP (b, c) and percentage of GFP-positive cells (d) were measured in both populations of cell. Shown are M ± SD, statistically significant (p < 0.05) differences are indicated by asterisks

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BMC Immunology

ISSN: 1471-2172

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