Fig. 1

The direct induction of IL-10 secreting B cells by EgPSC-ESPs in vitro. Splenic CD19⁺B cells were sorted from and cultured for 72 h in the presence of PBS, EgPSC-ESPs (5, 10 μg/ml), or LPS (10 μg/ml). The cells or supernatants were collected for further analysis. a Representative flow cytometry plots of IL-10⁺CD19⁺B cells post stimuli. b The statistic results of flow cytometry. c The relative expression of IL-10 mRNA in B cells. d The IL-10 production in culture supernatants. Data are expressed as means ± SD of triplicate wells in one round experiment (n = 6), and the results were repeated in three independent experiments. Differences were analyzed by one-way ANOVA with Bonferroni correction. VS PBS group, *P < 0.05; **P < 0.001. ESPs(5) VS ESP(10), ^#P < 0.05