Table 1 the real-time RT-PCR primers used in the study
Primer names | Sequences |
|---|---|
IL-10 | F: 5’-GCTCCAGAGCTGCGGACT-3’ |
R: 5’-TGTTGTCCAGCTGGTCCTTT-3’ | |
IL-6 | F: 5’-CCACGGCCTTCCCTAC-3’ |
R: 5’-AAGTGCATCATCGTTGT-3’ | |
TNF-α | F: 5’-CATCTTCTCAAAATTCGAGTGACAA-3’ |
R: 5’-TGGGAGTAGACAAGGTACAACCC-3’ | |
TLR-2 | F: 5’-TGTCTCCACAAGCGGGACTT-3’ |
R: 5’-TTCGATGGAATCGATGATGTTG-3’ | |
PTEN | F: 5’-AATTCCCAGTCAGAGGCGCTATGT-3’ |
R: 5’-GATTGCAAGTTCCGCCACTGAACA-3’ | |
PI3K | F: 5’-TCGGTCTGTAGATGAGGC-3’ |
R: 5’-CGGAGGAATGGATGAGGG-3’ | |
AKT | F: 5’-GTCGTCGCCAAGGATGAGG-3’ |
R: 5’-GGTCGTGGGTCTGGAATGA-3’ | |
TLR-9 | F: 5’-TGGCATGGCTACCTTTGCTAG-3’ |
R: 5’-AAATAGAGTCTTGCGGCTCCC-3’ | |
GAPDH | F: 5’-CAACTTTGGCATTGTGGAAGG-3’ |
R: 5’-ACACATTGGGGGTAGGAACAC-3’ |