Fig. 4

Production of IL-17A by CD4⁺ T helper cells. IL-17A–EGFP reporter mice (n = 4 per group) were uninfected (mock) or intranasally inoculated with 1 × 10⁵ cells with C. neoformans H99 strain (Cn H99), BALF, mLN and spleen were collected after 14 days for analysis. a A representative flow cytometrical plot of GFP⁺ cells in BALF, mLN and spleen among CD4⁺–gated T cells. % denotes the percentage of GFP⁺ cells appear inside the gated area. b–d Number of GFP⁺ cells among the CD4⁺– or CD8⁺–gated T cell populations in the (b) BALF, (c) mLN and (d) spleen. *P < 0.05, ***P < 0.001, n.s.: not significant or P ≥ 0.05, by Mann-Whitney U test