Expression of ligands for activating natural killer cell receptors on cell lines commonly used to assess natural killer cell function

Table 1 Antibody/Chimeric Protein Panel Composition

NK cell Receptor ligand specificity	aNKR/iNKR	Clone	Source	Fluorochrome
Panel 1
ULBP-1	NKG2D	170818	R & D Systems	PerCP
ULBP-2/5/6	NKG2D	165903	R & D Systems	APC
ULBP-3	NKG2D	166510	R & D Systems	PE
MIC-A	NKG2D	159227	R & D Systems	APC
MIC-B	NKG2D	236511	R & D Systems	AlexaFluor 700
Panel 2
CD48	2B4	BJ40	BioLegend	PE
CD80	Unknown for NK cells	2D10	BioLegend	BV421
CD86	Unknown for NK cells	IT2.2	BioLegend	PE-Dazzle 594
CD112	DNAM-1	610603	R & D Systems	AlexaFluor 700
CD155	DNAM-1	SKII.4	BioLegend	PE-Cy7
Panel 3
ICAM-1	LFA-1	HCD54	BioLegend	Pacific Blue
ICAM-2	LFA-1	CBR-1C2/2	BioLegend	PE
Unknown	NKp30	NKp30-Fc	R & D Systems	Unconjugated^a
Unknown	NKp44	NKp44-Fc	R & D Systems	Unconjugated^a
Unknown	NKp46	NKp46-Fc	R & D Systems	Unconjugated^a
HLA Panel
HLA-F	KIR3DS1	3D11	BioLegend	Unconjugated^b
HLA-F (KIR3DS1-Fc)	KIR3DS1	–	R & D Systems	Unconjugated^a
HLA-E	NKG2A	3D12	BioLegend	Unconjugated^b

^aChimeric protein binding was detected using a polyclonal anti-human IgG (Fcγ-specific)-PE conjugated secondary antibody (BioSciences)
^b3D11 and 3D12 binding was detected using a polyclonal F(ab’)₂ anti-mouse IgG-APC conjugated secondary antibody (eBioscience)

ISSN: 1471-2172