Expression of ligands for activating natural killer cell receptors on cell lines commonly used to assess natural killer cell function

Tremblay-McLean, Alexandra; Coenraads, Sita; Kiani, Zahra; Dupuy, Franck P.; Bernard, Nicole F.

doi:10.1186/s12865-018-0272-x

Table 2 Mean fluorescence intensity (MFI) of activating NK receptor ligand staining on K562, .221 and CEM.NKr.CCR5 cells by specific antibodies and chimeric proteins

From: Expression of ligands for activating natural killer cell receptors on cell lines commonly used to assess natural killer cell function

Ligand ID	Cell Stained
	K562				.221				CEM.NKr.CCR5
	MFI^a	Range MFI	Ctl^b	Background subtracted MFI	MFI	Range MFI	Ctl	Background subtracted MFI	MFI	Range MFI	Ctl	Background subtracted MFI^g
Panel 1
ULBP-1	895	852, 935	586^c	309	389	382, 396	413^c	− 24	276	271, 281	201	75^c
ULBP-2/5/6	3779	3685, 4905	503^d	3276	93.5	85, 123	79^d	14.5	115	101, 127	24^d	90
ULBP-3	1480	1219, 1895	749^d	731	396	374, 437	379^d	17	191	164, 223	159^d	32
MIC-A	1065	923, 1192	357^d	707	710	587, 1212	196^d	514	170	164, 183	156^d	16
MIC-B	786	784, 793	204^d	514	667	468, 877	113^d	554	107	94, 118	40^d	67
Panel 2
CD48	1044	788, 2347	169.2^d	874.5	43,843	30,131, 54,430	299^d	43,544	10,684	5081, 16,047	90^d	10,594
CD80	8980.5	7293, 10,778	527^d	8453	45,424	25,478, 65,004	526^d	44,898	469	425, 807	449^d	20
CD86	355	317, 367	264^d	90	55,878	53,432, 56,184	22^d	55,856	85	52, 112	67^d	17
CD112	3211	2485, 3923	296^d	2915	1.5	0.1, 12.1	1.6^d	− 0.12	544	470, 675	270^d	234
CD155	6187.5	5368, 6955	135^d	6052	70.8	67, 73	51^d	19.8	45	42, 54	28^d	18
Panel 3
ICAM-1	5087	4656, 11,162	497^d	4590	16,875	11,852, 22,761	612^d	16,875	203	185, 220	117^d	86.5
ICAM-2	54,429	50,356, 57,335	227^d	54,201	60,331	44,271, 78,093	379^d	59,952	61,416	47,186, 87,979	103^d	61,313
NKp30	1173	1152, 1217	157^e	1016	182	165, 209	208^e	− 25	160	116, 189	78^e	82
NKp44	668	619, 836	157^e	511	406	373, 469	208^e	145	297	194, 350	78^e	218
NPp46	302	165, 324	157^e	145	299	197, 450	208^e	91	268	209, 314	78^e	190
HLA Panel
HLA-F (3D11)	10,690	7197, 13,867	1252^c,e	9438^f	5032	4905, 6218	236^c,e	4796^f	1710	1608, 1881	189^e	1521
HLA-F (KIR3DS1-Fc)	9630	6437, 12,587	416^c,e	9219^f	8093	5789, 9287	236^c,e	7856^f	2861	2578, 3600	187^e	3380
HLA-E	280	208, 359	12^e	267	171	99, 250	2.5^e	169	477	435, 690	48^e	428

^aMedian of 6 replicates, MFI – mean fluorescence intensity
^bCtl = Control staining
^cControl staining where control was an isotype control
^dControl staining where control was fluorescence minus one
^eControl staining where control was a fluorochrome conjugated secondary antibody alone
^fThe isotype control was used for background correction
^gAverage of 6 replicates

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ISSN: 1471-2172

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