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Table 2 Mean fluorescence intensity (MFI) of activating NK receptor ligand staining on K562, .221 and CEM.NKr.CCR5 cells by specific antibodies and chimeric proteins

From: Expression of ligands for activating natural killer cell receptors on cell lines commonly used to assess natural killer cell function

Ligand ID Cell Stained
K562 .221 CEM.NKr.CCR5
MFIa Range MFI Ctlb Background subtracted MFI MFI Range MFI Ctl Background subtracted MFI MFI Range MFI Ctl Background subtracted MFIg
Panel 1
 ULBP-1 895 852, 935 586c 309 389 382, 396 413c − 24 276 271, 281 201 75c
 ULBP-2/5/6 3779 3685, 4905 503d 3276 93.5 85, 123 79d 14.5 115 101, 127 24d 90
 ULBP-3 1480 1219, 1895 749d 731 396 374, 437 379d 17 191 164, 223 159d 32
 MIC-A 1065 923, 1192 357d 707 710 587, 1212 196d 514 170 164, 183 156d 16
 MIC-B 786 784, 793 204d 514 667 468, 877 113d 554 107 94, 118 40d 67
Panel 2
 CD48 1044 788, 2347 169.2d 874.5 43,843 30,131, 54,430 299d 43,544 10,684 5081, 16,047 90d 10,594
 CD80 8980.5 7293, 10,778 527d 8453 45,424 25,478, 65,004 526d 44,898 469 425, 807 449d 20
 CD86 355 317, 367 264d 90 55,878 53,432, 56,184 22d 55,856 85 52, 112 67d 17
 CD112 3211 2485, 3923 296d 2915 1.5 0.1, 12.1 1.6d − 0.12 544 470, 675 270d 234
 CD155 6187.5 5368, 6955 135d 6052 70.8 67, 73 51d 19.8 45 42, 54 28d 18
Panel 3
 ICAM-1 5087 4656, 11,162 497d 4590 16,875 11,852, 22,761 612d 16,875 203 185, 220 117d 86.5
 ICAM-2 54,429 50,356, 57,335 227d 54,201 60,331 44,271, 78,093 379d 59,952 61,416 47,186, 87,979 103d 61,313
 NKp30 1173 1152, 1217 157e 1016 182 165, 209 208e − 25 160 116, 189 78e 82
 NKp44 668 619, 836 157e 511 406 373, 469 208e 145 297 194, 350 78e 218
 NPp46 302 165, 324 157e 145 299 197, 450 208e 91 268 209, 314 78e 190
HLA Panel
 HLA-F (3D11) 10,690 7197, 13,867 1252c,e 9438f 5032 4905, 6218 236c,e 4796f 1710 1608, 1881 189e 1521
 HLA-F (KIR3DS1-Fc) 9630 6437, 12,587 416c,e 9219f 8093 5789, 9287 236c,e 7856f 2861 2578, 3600 187e 3380
 HLA-E 280 208, 359 12e 267 171 99, 250 2.5e 169 477 435, 690 48e 428
  1. aMedian of 6 replicates, MFI – mean fluorescence intensity
  2. bCtl = Control staining
  3. cControl staining where control was an isotype control
  4. dControl staining where control was fluorescence minus one
  5. eControl staining where control was a fluorochrome conjugated secondary antibody alone
  6. fThe isotype control was used for background correction
  7. gAverage of 6 replicates