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Fig. 1 | BMC Immunology

Fig. 1

From: A novel approach for rapid high-throughput selection of recombinant functional rat monoclonal antibodies

Fig. 1

The schematic illustration of a rapid high-throughput method to select recombinant RtmAbs. Rats were immunized with pooled cells consisting of different antigen-expressing cells. Hybridomas were generated by using rat spleen-mouse myeloma fusions, and supernatants were screened for positive clones using indirect cell based ELISA. The rearranged heavy and light chain variable regions were amplified from RNA extracted from hybridoma cells by RT-PCR and cloned into a single expression plasmid. The recombinant RtmAbs were produced by transiently transfecting mammalian cells

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