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Table 1 Primers used for the generation of recombinant rat monoclonal antibodies

From: A novel approach for rapid high-throughput selection of recombinant functional rat monoclonal antibodies

Reverse primer used for the amplification of heavy chain variable regions

Reverse primer used for the amplification of κ light chain variable regions

 R1: AGGATCCAGGRRCCARKGGATAGACAGATG

R2: AGGAGATGGTGGGAAGATGGATACAGTTG

Degenerate primer used for the amplification of heavy chain variable regions

Degenerate primer used for the amplification of κ light chain variable regions

hF:

κF:

 1. GCCCGTTTCTGCTAGC ATGTTGGTSCTGMAGTKGGTTTTSGTG

1. GCAATCCAGGTCCA ATGAAAATGACGACACCTGCTCAGTTC

 2. GCCCGTTTCTGCTAGC ATGAGACTACTAGGTCTTCTCCTGTGC

2. GCAATCCAGGTCCA ATGAGGGCCCCTTTTCAGTTACTTGGG

 3. GCCCGTTTCTGCTAGC ATGGGATGGAGCCAGATCATCCTCTTT

3. GCAATCCAGGTCCA ATGTCTAAAAACTTATTAGAAGTTTCA

 4. GCCCGTTTCTGCTAGC ATGGTTTTCGGGCTGATCCTTTTTCTT

4. GCAATCCAGGTCCA ATGGTAAGTCCTGCCCAGTTCCTGTTT

 5. GCCCGTTTCTGCTAGC ATGATGGGTTTAGGGGTGATTCTTTTT

5. GCAATCCAGGTCCA ATGATGGCTGCAGTTCAACTCTTAGGG

 6. GCCCGTTTCTGCTAGC ATGGGATTGAGCTGGGTTTTTCTTGTT

6. GCAATCCAGGTCCA ATGAAAGTGCCTGGTAGGCTGCTGGTG

 7. GCCCGTTTCTGCTAGC ATGGACTTGCGACTGACTTATGTCTTT

7. GCAATCCAGGTCCA ATGAAGTGGCTGTTAGTCTGTTGGTGC

 8. GCCCGTTTCTGCTAGC ATGACTATCCTGGTGCTTCTCCTCTGT

8. GCAATCCAGGTCCA ATGATTCCTGCCCAGTTCCTGTTTCTG

 9. GCCCGTTTCTGCTAGC ATGGCTRTCCTGGTGCTGTTGCTCTGC

9. GCAATCCAGGTCCA ATGATGAGTCCTGCCCAGTTCCTGTTT

 10. GCCCGTTTCTGCTAGC ATGRAATGSARCTGGRTCWTYYTCTTY

10. GCAATCCAGGTCCA ATGAATTTTCAGGTGCAGGTTTTTAGC

 11. GCCCGTTTCTGCTAGC ATGGCTGTCCTGGTGCTGTTGCTCTGC

11. GCAATCCAGGTCCA ATGAAYGTGYCCACTCAACTCCTTGGG

 12. GCCCGTTTCTGCTAGC ATGAGARYGYYGRKTCTTCTGTACCTG

12. GCAATCCAGGTCCA ATGGACATGAGGGCCCATRCTCAGTTT

 13. GCCCGTTTCTGCTAGC ATGGACWTCAGNCTCAGCTTGGBTTTC

13. GCAATCCAGGTCCA ATGARRKTYSNBVYTSAGYTTYKKGGG

 14. GCCCGTTTCTGCTAGC ATGRAGTTGDGSMTRANCTGGRTTTTY

Primer used for the amplification of heavy and light chains

 F3: CATCTGTCTATCCACTGGCTCCTGGAAC

R3: TGACAGGTGCGCGTTTAGCACG TCATTTACCCGGAGAGTGGGAGAG

 F4: CGTGCTAAACGCGCACCTGTCAAAC

R4: TGGACCTGGATTGCTTTCTACATC

 F5: CAACTGTATCCATCTTCCCACCATC

R5: GATCCGGCCTTGCCGGC CCTAACACTCATTCCTGTTGAAG

  1. The primers used to amplify the heavy and κ light chain were designed using the genome sequence of the rat immunoglobulin: GenBank: DQ402471.1 for the κ light chain and GenBank: BC095846.1 for the heavy chain. Degenerate base code: M = A + C; R = A + G; W = A + T; S = C + G; Y = C + T; K = G + T; V = A + C + G; B = C + G + T; D = G + A + T; N = C + G + A + T
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BMC Immunology

ISSN: 1471-2172

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