Fig. 2
From: 5-Aminolevulinic acid regulates the immune response in LPS-stimulated RAW 264.7 macrophages
ALA significantly reduced LPS-induced iNOS mRNA and protein expression. RAW264.7 was incubated in 1 μg/mL LPS condition with or without 1 mM ALA. After incubation, a iNOS mRNA levels were analyzed by Real-time PCR (Values are means ± SD, n = 3) and b iNOS protein levels were analyzed by Western blotting (Upper panel, representative Western blot bands. Lower panel, summarized bar graph shows band intensity presented as ratio of iNOS over GAPDH. Values are means ± SD, n = 2, p values are shown in figures)