Fig. 7

Human immune cells expansion and phenotype changes in NSG-cmah^−/− mice. Males NSG-cmah^−/− and wt NSG 5 weeks of age were transplanted intraperitoneally with single-donor human peripheral blood mononuclear cells. The number and phenotype of human cells in peripheral blood were analyzed up to 31 days post transplantation and percentage of human cells in the spleen at the end point of observation. a Gating strategy to identify human CD45⁺ cells, CD19⁺, CD14⁺, and CD3⁺ T cells and their subpopulations. b Absolute counts of human cells in peripheral blood with accelerated expansion of human T cells and CD4⁺ cells in the NSG-cmah−/− compared to NSG. c Proportion of human cells in the spleens at the end-point were similar in both strains. d Proportion of memory CD45RO⁺ and central memory CD45RO⁺CD62L⁺ were increased in NSG-cmah^−/− mice among CD4⁺ T-cells in blood and spleen. e Proportion of CD8⁺ T-cells were also increased but to a lesser degree. Six animals per group (males) were used and b and d represent means with SEM, c and e individual mouse data. Statistical analysis done by 2-way ANOVA with Sidak’s multiple comparison test, * - P < 0.05