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Fig. 2 | BMC Immunology

Fig. 2

From: Active immunization with norovirus P particle-based amyloid-β chimeric protein vaccine induces high titers of anti-Aβ antibodies in mice

Fig. 2

Production and purification of His-tagged Aβ1–6 chimeric protein. (a) Ni-NTA affinity purification of the His-tagged Aβ1–6 chimeric SP. Target protein was eluted with 200 mM imidazole. (b) Size-exclusion chromatography of the His-tagged Aβ1–6 chimeric SP using a Superdex 200 prep grade column. (c) SDS-PAGE, Native-PAGE, and anti-His-tag western blot analysis of SP and IB of His-tagged Aβ1–6 chimeric proteins

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BMC Immunology

ISSN: 1471-2172

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