Fig. 1

Differential gene expression in response to ZFP36 overexpression. a HeLa cells were transiently transfected with either a ZFP36-overexpressing or control vector followed by determination of the level of FLAG-tagged TTP protein expression by western blot using an antibody against FLAG. b and c The FPKM values were calculated as described in the Methods. The level of mRNA expression was measured by qRT-PCR and RNA-seq. d Detection of the TTP- regulated genes on the volcano plots, up-regulated genes (FC ≥ 1.5; FDR < 0.05) are labeled red, whereas down-regulated (FC ≤ 2/3; FDR < 0.05) genes are labeled blue