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Fig. 3 | BMC Immunology

Fig. 3

From: Identification and characterisation of transient receptor potential melastatin 2 and CD38 channels on natural killer cells using the novel application of flow cytometry

Fig. 3

Dual identification of TRPM2 and CD38 surface expression on Natural Killer cell subsets. TRPM2/CD38 surface expression was measured on (a) CD56DimCD16+ and (b) CD56BrightCD16Dim/− NK cells by flow cytometry. TRPM2/CD38 surface expression was comparatively measured at 2 h/1 h [black] and 1 h/30 min [grey] following 1:300, 1:100, 1:50, 1:10 and 1:5 primary TRPM2 antibody dilutions. Representative flow cytometry dot plots of each respective dilution are presented in Additional file 4: Figure S4, Additional file 5: Figure S5, Additional file 6: Figure S6, Additional file 7: Figure S7, Additional file 8: Figure S8, Additional file 9: Figure S9, Additional file 10: Figure S10, Additional file 11: Figure S11, Additional file 12: Figure S12, Additional file 13: Figure S13 and Additional file 17: Figure S17, Additional file 18: Figure S18, Additional file 19: Figure S19, Additional file 20: Figure S20, Additional file 21: Figure S21, Additional file 22: Figure S22, Additional file 23: Figure S23, Additional file 24: Figure S24, Additional file 25: Figure S25, Additional file 26: Figure S26. Bar graphs report the means ± SEM. Mann Whittney U test and Kruskal Wallis H test were performed. * p < 0.05, ** p < 0.01. 1° primary, TRPM2, Transient Receptor Potential Melastatin 2

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