Fig. 2

MHCII-KO mice fail to generate allergic airway inflammation to OVA or HDM. (a–d) C57BL/6 and MHCII-KO mice were immunized and challenged with OVA or HDM as in Fig. 1, PBS refers to C57BL/6 mice that were mock-immunized and challenged with OVA or HDM. Total cell counts, percent and number of eosinophils were evaluated by flow cytometry; bar graphs show mean + SEM/mouse from two combined experiments each using 5–8 mice per group. Histopathological scores were calculated on 6–10 mice per condition, selected from both experiments. Peribronchiolar and perivascular inflammation scores are displayed as median +/− interquartile range; the percent PAS-positive staining in bronchiolar epithelial cells is shown as mean + SEM. P values refer to comparisons between the sensitised and challenged C57BL/6 and MHCII-KO groups. ***, p < 0.001; **, p < 0.01; *, p < 0.05; ns, not significant