Fig. 6

Immunohistochemical visualizations of middle ear (ME) and bone marrow (BM) sections showing the presence of L-selectin in the BM but not in ME infiltrating leukocytes (primarily neutrophils). This indicates that L-selectin is shed from the activated leukocytes as they enter the ME after NTHi infection. The right panel shows sections reacted without the primary antibody as a negative control. Original magnification 400x