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Table 1 Impaired bacterial clearance of middle ears in mice with CD44 deficiency

From: Lack of the hyaluronan receptor CD44 affects the course of bacterial otitis media and reduces leukocyte recruitment to the middle ear

Time after NTHi inoculation C57BL/6 WT culture positive plates C57BL/6 WT mean NTHi DS C57BL/6 WT CFU/mL CD44 −/− culture positive plates CD44 −/− mean NTHi DS CD44 −/− CFU/mL
Day 1 6/6 (100%) 3.20 6 × 104 4/6 (67%) 1.70 6 × 104
Day 2 3/6 (50%) 1.30 1 × 104 4/6 (67%) 1.80 2 × 105
Day 3 1/6 (17%) 0.50 1 × 103 2/6 (33%) 1.30 2 × 105
Day 7 0/8 (0%) 0.00 0 1/8 (13%) 0.50 1 × 105
Day 10 0/6 (0%) 0.00 0 1/6 (17%) 0.70 8 × 104
  1. Bacterial colonization of chocolate-agar plates was evaluated using quantitatively and semi-quantitative methods to generate an NTHi detection score (DS): 0 indicated no colony-forming unit (CFU) on the plate; 1 indicated CFUs in one quadrant; 2 indicated CFUs in two quadrants; 3 indicated CFUs in three quadrants; and 4 indicated CFUs in all four quadrants on the plate. The colonies on the 4 quadrants were also counted and multiplied by 103 to provide a CFU count per mL. At least 6 ears (n ≥ 3 mice) were cultured for each time points of WT and CD44−/− mice. NTHi clearance by CD44 KO animals was significantly worse than for C57/BL6 controls by ANOVA (p = 0.0114)