Fig. 2

Respiratory burst and degranulation in macrophage migration inhibitory factor (MIF)-primed neutrophils induced by membrane proteinase-3 (PR3)-antineutrophil cytoplasmic antibody (ANCA)-positive immunoglobulin G (IgG) or myeloperoxidase (MPO)-ANCA-positive IgG. Respiratory burst induced by patient-derived MPO-ANCA-positive IgG (a) or PR3-ANCA-positive IgG (b) in MIF-primed and non-primed neutrophils obtained from five healthy donors, respectively, was reflected by dihydrorhodamine conversion into rhodamine-123. MFI, mean fluorescence intensity. Neutrophil degranulation induced by patient-derived MPO-ANCA-positive IgG (c) or PR3-ANCA-positive IgG (d) was measured in MIF-primed and non-primed neutrophils obtained from five healthy donors. Bars represent mean ± standard deviation of measurements made in five independent experiments