Fig. 5
A macrophage migration inhibitory factor (MIF) antagonist inhibits respiratory burst and degranulation in neutrophils primed with culture supernatants from C5a-stimulated neutrophils and administered antineutrophil cytoplasmic antibody (ANCA). a Respiratory burst in neutrophils (obtained from five healthy donors) under various experimental conditions measured from dihydrorhodamine-123 conversion into rhodamine-123. DMSO, dimethyl sulfoxide (control); C5a-supernatant, supernatant from neutrophils primed with C5a; ISO-1, (S,R)3-(4-hydroxyphenyl)-4,5-dihydro-5-isoxazole acetic acid methyl ester (MIF antagonist); MFI, mean fluorescence intensity; MPO-ANCA-positive IgG, patient-derived myeloperoxidase-ANCA-positive immunoglobulin G; PR3-ANCA-positive IgG, patient-derived membrane proteinase-3-ANCA-positive immunoglobulin G. b Degranulation of neutrophils (obtained from five healthy donors) under various experimental conditions, determined from measurements of lactoferrin levels in culture supernatants. DMSO, dimethyl sulfoxide (control); C5a-supernatant, supernatant from neutrophils primed with C5a; ISO-1, (S,R)3-(4-hydroxyphenyl)-4,5-dihydro-5-isoxazole acetic acid methyl ester (MIF antagonist); MPO-ANCA-positive IgG, patient-derived myeloperoxidase-ANCA-positive immunoglobulin G; PR3-ANCA-positive IgG, patient-derived membrane proteinase-3-ANCA-positive immunoglobulin G. Bars represent mean ± standard deviation of measurements made in 5 independent experiments and donors