Table 6 Suppliers of biomarker assays, minimum detectable dose (MDD), Intra-assay coefficient of variation (CV %), and mean concentration of biomarker sample #1 and 2 of the kits
Supplier of biomarkers | MDD | Intra-Assay Precision | Assayed | ||
|---|---|---|---|---|---|
A. | Cytokines/Chemokines | Sample #1 (CV%, mean) | Sample #2 (CV%, mean) | ||
1 | Interleukin-1ß (IL-1 ß)a | 0.033 pg/mL | 4.4, 0.315 pg/mL (n = 20) | 3.9, 1.30 pg/mL (n = 20) | singleton |
1 | Interleukin-6 (IL-6)a | 0.039 pg/mL | 6.9, 0.436 pg/mL (n = 20) | 7.8, 2.45 pg/mL (n = 20) | duplicate |
2 | Interleukin-12 (IL-12p70) | 2.1 pg/mL | 5.0, 15.85 pg/mL (n = 8) | 2.13, 45.0 pg/mL (n = 8) | duplicate |
1 | IL-1 receptor antagonist | 6.3 pg/mL | 7.3, 66.9 pg/mL (n = 20) | 5.0, 607 pg/mL (n = 20) | duplicate |
2 | Interferon-γ (IFN-γ) | 0.03 IU/mL | 3.2, 1.26 IU/mL (n = 20) | 3.8, 12.28 IU/mL (n = 20) | duplicate |
2 | Tumor Necrosis Factor-α | 3.0 pg/mL | 5.2, 86.7 pg/mL (n = 20) | 3.7, 591 pg/mL (n = 20) | duplicate |
1 | Interleukin-8 (IL-8)a | 0.13 pg/mL | 5.5, 5.5 pg/mL (n = 20) | 7.3, 37.1 pg/mL(n = 20) | singleton |
1 | CCL3 (MIP-1 α) | 10.0 pg/mL | 8.9, 140 pg/mL (n = 20) | 8.8, 688 pg/mL (n = 20) | duplicate |
1 | CCL4 (MIP-1 β) | 11.0 pg/mL | 9.0, 51.3 pg/mL (n = 20) | 3.6, 208 pg/mL (n = 20) | duplicate |
1 | CCL5 (RANTES) | 2.0 pg/mL | 2.5, 91.9 pg/mL (n = 20) | 1.7, 573 pg/mL (n = 20) | duplicate |
B. | Adipocytokines | ||||
1 | Adiponectin | 0.25 ng/mL | 2.5, 19.8 ng/mL (n = 20) | 3.4, 69.9 ng/mL (n = 20) | duplicate |
1 | Leptin | 7.8 pg/mL | 3.3, 64.5 pg/mL (n = 20) | 3.0, 146 pg/mL (n = 20) | duplicate |
C. | Soluble Receptors markers | ||||
1 | sCD14 | 125 pg/mL | 6.4, 1111 pg/mL (n = 20) | 4.8, 2158 pg/mL (n = 20) | duplicate |
1 | sCD25 (sIL-2Ra) | 125 pg/mL | 6.1, 207 pg/mL (n = 20) | 6.1, 613 pg/mL (n = 20) | duplicate |
1 | sCD40L | 10 pg/mL | 5.1, 430 pg/mL (n = 20)) | 4.5, 1212 pg/mL (n = 20) | duplicate |
1 | sCD120b (sTNF-RII) | 4.2 pg/mL | 3.2, 68.7 pg/mL (n = 20) | 2.6, 179 pg/mL (n = 20) | duplicate |
1 | sCD126 (sIL-6R) | 0.6 pg/mL | 8.6, 134 pg/mL (n = 20) | 2.6, 644 pg/mL (n = 20) | singleton |
1 | sCD130 (spg130) | 6.5 pg/mL | 4.3, 0.70 ng/mL (n = 20) | 5.5, 1.82 ng/mL (n = 20) | duplicate |
1 | sCD163 | 0.08 ng/mL | 3.8, 20.0 ng/mL (n = 20) | 3.4, 35.1 ng/mL (n = 20) | duplicate |
D. | Immune Activation markers | ||||
3 | Neopterin | 2.0 nmol/L | 7.4, 4.5 nmol/L (n = 10) | 5.6, 18.7 nmol/L (n = 10) | duplicate |