Fig. 2

Mø content (%) in cells segregated by adhesion to bacteriological Petri dishes assessed by the phagocytosis of fluorescent beads. Cells adherent to the Petri dish were incubated with fluorescent beads, with an average diameter of 1.0 μm, for 2 h and then fixed. Phase-contrast images and green fluorescence images of the same fields were captured. a: Representative fluorescence images merged with phase-contrast images showing fluorescent beads phagocytosed by liver, spleen, lung, and brain Mø propagated by co-culture and subculture. b: More than 700 cells per sample were calculated, and the percentage of Mø in each organ (liver, spleen, lung, brain) derived from more than four mice was determined from more than four independent experiments. Bar graphs showing the percent content of Mø as the mean ± SD (liver, 98.8 ± 0.4; spleen, 99.2 ± 0.9; Lung, 97.6 ± 1.3; brain 98.8 ± 0.7)