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Fig. 7 | BMC Immunology

Fig. 7

From: Simple propagation method for resident macrophages by co-culture and subculture, and their isolation from various organs

Fig. 7

Morphology and expression levels of M1 and M2 polarisation markers in spleen Mø treated with M1 and M2 polarisation inducers. Spleen macrophages were treated with or without (control) LPS plus IFN-γ (M1 inducer) and IL-4 (M2 inducer) for 24 h. a: Representative phase-contrast images of spleen Mø treated with or without the inducers. b: Representative histograms from flow cytometric analyses, showing the expression of CD11b (pan Mø marker), CD11c and CD68 (M1 polarisation markers for monocyte-derived Mø), and CD206 (M2 polarisation marker for monocyte-derived Mø)

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