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Table 1 ELISA validity parameters for the detection of IgA and IgG antibodies against mycobacterial antigens MT10.3, MPT64, PPE59, and the MT10.3:MPT64 and the F2 fusion proteins, at different pleural fluid (PF) and serum dilutions

From: IgA and IgG antibody detection of mycobacterial antigens in pleural fluid and serum from pleural tuberculous patients

ELISA tests (samples dilutions)

PL-TB (n = 29)

OPL (n = 39)

p valora

Cut off

AUC

Sensitivity (%)

(Number of +)

Specificity (%)

(Number of -)

Mean ± SD

Mean ± SD

IgA-(PF)

 MT10.3 (1:100)

0.983 ± 0.336

0.548 ± 0.321

*

1.278

0.852

20.7 (6)

95 (37)

 MPT64 (1:100)

1.164 ± 0.350

0.606 ± 0.352

*

1.505

0.887

24.1 (7)

95 (37)

 MT10.3-MPT64 (1:50)

0.821 ± 0.213

0.387 ± 0.188

*

0.658

0.949

86.2 (25)

95 (37)

 MT10.3-MPT64 (1:100)

0.667 ± 0.195

0.302 ± 0.138

*

0.598

0.950

65.5 (19)

95 (37)

 MT10.3-MPT64 (1:200)

0.542 ± 0.154

0.232 ± 0.093

*

0.385

0.968

86.2 (25)

95 (37)

 MT10.3-MPT64 (1:400)

0.433 ± 0.126

0.185 ± 0.060

*

0.315

0.980

82.8(24)

95 (37)

 MT10.3-MPT64 (1:800)

0.323 ± 0.125

0.209 ± 0.094

*

0.404

0.823

27.6 (8)

95 (37)

 PPE59 (1:100)

0.696 ± 0.186

0.392 ± 0.155

*

0.639

0.904

62.1 (18)

95 (37)

 F2 (1:50)

0.553 ± 0.230

0.351 ± 0.212

*

0.542

0.774

44.8 (13)

95 (37)

IgA-(serum)

 MT10.3 (1:50)

0.996 ± 0.282

0.865 ± 0.279

0.081

1.282

0.625

20.7 (6)

95 (37)

 MT10.3 (1:100)

0.888 ± 0.281

0.714 ± 0.228

0.012

1.018

0.678

27.6 (8)

95 (37)

 MT10.3 (1:200)

0.760 ± 0.263

0.634 ± 0.209

0.057

0.962

0.636

13.8 (4)

95 (37)

 MT10.3 (1:400)

0.622 ± 0.216

0.528 ± 0.172

0.130

0.799

0.608

13.8 (4)

95 (37)

 MPT64 (1:50)

1.109 ± 0.341

0.843 ± 0.371

0.003

1.462

0.708

20.7 (6)

95 (37)

 MPT64 (1:100)

1.045 ± 0.323

0.757 ± 0.308

*

1.220

0.729

27.6 (8)

95 (37)

 MPT64 (1:200)

0.959 ± 0.347

0.693 ± 0.258

0.002

1.048

0.717

34.5 (10)

95 (37)

 MPT64 (1:400)

0.860 ± 0.338

0.630 ± 0.231

0.006

1.012

0.695

27.6 (8)

95 (37)

 MT10.3:MPT64 (1:50)

1.176 ± 0.338

0.621 ± 0.282

*

1.267

0.905

44.8 (13)

95 (37)

 PPE59 (1:50)

0.868 ± 0.204

0.706 ± 0.206

*

1.042

0.736

13.8 (4)

95 (37)

 PPE59 (1:100)

0.841 ± 0.189

0.663 ± 0.190

*

1.043

0.785

13.8 (4)

95 (37)

 F2 (1:25)

0.963 ± 0.304

0.624 ± 0.195

*

0,957

0.837

51.7 (15)

95 (37)

 F2 (1:50)

0.810 ± 0.271

0.507 ± 0.165

*

0,772

0.844

51.7 (15)

95 (37)

 F2 (1:100)

0.698 ± 0.282

0.414 ± 0.134

*

0,670

0.847

48.3 (14)

95 (37)

 F2 (1:200)

0.619 ± 0.252

0.443 ± 0.188

*

0,591

0.879

44.8 (13)

95 (37)

IgG-(PF)

 MT10.3-MPT64 (1:200)

0.840 ± 0.352

0.347 ± 0.126

*

0.616

0.952

65.5 (19)

95 (37)

IgG-(serum)

 MT10.3:MPT64 (1:50)

0.863 ± 0.196

0.636 ± 0.195

*

0.891

0.809

51.7 (15)

95 (37)

 MT10.3 (1:400)

1.409 ± 0.218

1.190 ± 0.261

*

1.641

0.739

17.2 (5)

95 (37)

 MPT64 (1:400)

1.256 ± 0.201

1.173 ± 0.213

0.129

1.490

0.609

10.3 (3)

95 (37)

 PPE59 (1:50)

0.918 ± 0.252

0.789 ± 0.327

0.204

1.370

0.591

3.4 (1)

95 (37)

 PPE59 (1:100)

0.898 ± 0.346

0.709 ± 0.279

0.003

1.181

0.711

17.2 (5)

95 (37)

 F2 (1:200)

0.604 ± 0.319

0.499 ± 0.313

0.087

1.084

0.622

6.9 (2)

95 (37)

 F2 (1400)

0.467 ± 0.267

0.387 ± 0.232

0.127

0.787

0.609

10.3 (3)

95 (37)

 F2 (1:800)

0.369 ± 0.180

0.289 ± 0.161

0.024

0.552

0.660

13.8 (4)

95 (37)

 F2 (1:1600)

0.271 ± 0.127

0.223 ± 0.123

0.031

0.427

0.663

10.3 (3)

95 (37)

  1. IgA and IgG mean level results in optical density and their standard deviations (SD)
  2. PLTB pleural tuberculosis patients, OPL patients with other, non-TB, pleural diseases, AUC area under the curve
  3. aSignificant difference of IgA/IgG production between groups and between tested antigen were calculated by the Mann Whitney test (p < 0.001*)
  4. Boldface: represents the antigens tests of best sensitivities
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BMC Immunology

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