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Table 1 ELISA validity parameters for the detection of IgA and IgG antibodies against mycobacterial antigens MT10.3, MPT64, PPE59, and the MT10.3:MPT64 and the F2 fusion proteins, at different pleural fluid (PF) and serum dilutions

From: IgA and IgG antibody detection of mycobacterial antigens in pleural fluid and serum from pleural tuberculous patients

ELISA tests (samples dilutions) PL-TB (n = 29) OPL (n = 39) p valora Cut off AUC Sensitivity (%)
(Number of +)
Specificity (%)
(Number of -)
Mean ± SD Mean ± SD
IgA-(PF)
 MT10.3 (1:100) 0.983 ± 0.336 0.548 ± 0.321 * 1.278 0.852 20.7 (6) 95 (37)
 MPT64 (1:100) 1.164 ± 0.350 0.606 ± 0.352 * 1.505 0.887 24.1 (7) 95 (37)
MT10.3-MPT64 (1:50) 0.821 ± 0.213 0.387 ± 0.188 * 0.658 0.949 86.2 (25) 95 (37)
 MT10.3-MPT64 (1:100) 0.667 ± 0.195 0.302 ± 0.138 * 0.598 0.950 65.5 (19) 95 (37)
 MT10.3-MPT64 (1:200) 0.542 ± 0.154 0.232 ± 0.093 * 0.385 0.968 86.2 (25) 95 (37)
 MT10.3-MPT64 (1:400) 0.433 ± 0.126 0.185 ± 0.060 * 0.315 0.980 82.8(24) 95 (37)
 MT10.3-MPT64 (1:800) 0.323 ± 0.125 0.209 ± 0.094 * 0.404 0.823 27.6 (8) 95 (37)
PPE59 (1:100) 0.696 ± 0.186 0.392 ± 0.155 * 0.639 0.904 62.1 (18) 95 (37)
 F2 (1:50) 0.553 ± 0.230 0.351 ± 0.212 * 0.542 0.774 44.8 (13) 95 (37)
IgA-(serum)
 MT10.3 (1:50) 0.996 ± 0.282 0.865 ± 0.279 0.081 1.282 0.625 20.7 (6) 95 (37)
 MT10.3 (1:100) 0.888 ± 0.281 0.714 ± 0.228 0.012 1.018 0.678 27.6 (8) 95 (37)
 MT10.3 (1:200) 0.760 ± 0.263 0.634 ± 0.209 0.057 0.962 0.636 13.8 (4) 95 (37)
 MT10.3 (1:400) 0.622 ± 0.216 0.528 ± 0.172 0.130 0.799 0.608 13.8 (4) 95 (37)
 MPT64 (1:50) 1.109 ± 0.341 0.843 ± 0.371 0.003 1.462 0.708 20.7 (6) 95 (37)
 MPT64 (1:100) 1.045 ± 0.323 0.757 ± 0.308 * 1.220 0.729 27.6 (8) 95 (37)
 MPT64 (1:200) 0.959 ± 0.347 0.693 ± 0.258 0.002 1.048 0.717 34.5 (10) 95 (37)
 MPT64 (1:400) 0.860 ± 0.338 0.630 ± 0.231 0.006 1.012 0.695 27.6 (8) 95 (37)
 MT10.3:MPT64 (1:50) 1.176 ± 0.338 0.621 ± 0.282 * 1.267 0.905 44.8 (13) 95 (37)
 PPE59 (1:50) 0.868 ± 0.204 0.706 ± 0.206 * 1.042 0.736 13.8 (4) 95 (37)
 PPE59 (1:100) 0.841 ± 0.189 0.663 ± 0.190 * 1.043 0.785 13.8 (4) 95 (37)
F2 (1:25) 0.963 ± 0.304 0.624 ± 0.195 * 0,957 0.837 51.7 (15) 95 (37)
F2 (1:50) 0.810 ± 0.271 0.507 ± 0.165 * 0,772 0.844 51.7 (15) 95 (37)
 F2 (1:100) 0.698 ± 0.282 0.414 ± 0.134 * 0,670 0.847 48.3 (14) 95 (37)
 F2 (1:200) 0.619 ± 0.252 0.443 ± 0.188 * 0,591 0.879 44.8 (13) 95 (37)
IgG-(PF)
MT10.3-MPT64 (1:200) 0.840 ± 0.352 0.347 ± 0.126 * 0.616 0.952 65.5 (19) 95 (37)
IgG-(serum)
MT10.3:MPT64 (1:50) 0.863 ± 0.196 0.636 ± 0.195 * 0.891 0.809 51.7 (15) 95 (37)
 MT10.3 (1:400) 1.409 ± 0.218 1.190 ± 0.261 * 1.641 0.739 17.2 (5) 95 (37)
 MPT64 (1:400) 1.256 ± 0.201 1.173 ± 0.213 0.129 1.490 0.609 10.3 (3) 95 (37)
 PPE59 (1:50) 0.918 ± 0.252 0.789 ± 0.327 0.204 1.370 0.591 3.4 (1) 95 (37)
 PPE59 (1:100) 0.898 ± 0.346 0.709 ± 0.279 0.003 1.181 0.711 17.2 (5) 95 (37)
 F2 (1:200) 0.604 ± 0.319 0.499 ± 0.313 0.087 1.084 0.622 6.9 (2) 95 (37)
 F2 (1400) 0.467 ± 0.267 0.387 ± 0.232 0.127 0.787 0.609 10.3 (3) 95 (37)
 F2 (1:800) 0.369 ± 0.180 0.289 ± 0.161 0.024 0.552 0.660 13.8 (4) 95 (37)
 F2 (1:1600) 0.271 ± 0.127 0.223 ± 0.123 0.031 0.427 0.663 10.3 (3) 95 (37)
  1. IgA and IgG mean level results in optical density and their standard deviations (SD)
  2. PLTB pleural tuberculosis patients, OPL patients with other, non-TB, pleural diseases, AUC area under the curve
  3. aSignificant difference of IgA/IgG production between groups and between tested antigen were calculated by the Mann Whitney test (p < 0.001*)
  4. Boldface: represents the antigens tests of best sensitivities