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Fig. 8 | BMC Immunology

Fig. 8

From: Subsets of mononuclear phagocytes are enriched in the inflamed colons of patients with IBD

Fig. 8

Augmentation of macrophages and dendritic cell subsets in mouse colon following chronic DSS stimulation. a Schematic representation of chronic DSS experiment. b Flow Cytometry gating for macrophages and dendritic cell subsets studied (shown for colonic LP). For pDCs, the pre-gating was Viable>CD45 + Epcam- > CD3-CD19- > CD11c + CD11b- > Ly6c + B220+ events; For macrophages and conventional dendritic cells, the pre-gating was Viable>CD45 + Epcam- > CD3-CD19->. Dendritic cells studied are total conventional dendritic cells or tcDCs (CD11c + MHCII+F4/80-); cDC subsets: DC1 (CD103 + CD11b-), DC2 (CD103-CD11b+) and double positive DC1, 2 (CD103 + CD11b+); and pDCs (SiglecH+Ly6c + B220 + CD11b-CD11c+). Macrophages studied are total macrophages or tMACs (CD103-F4/80 + CD11b+); CD11chi MHCII+ subset and CD11clo MHCII+ ones. c Changes in macrophage and dendritic cell frequencies (reported as amongst total hematopoietic cells) following chronic DSS stimulation in colonic IEL (left panel) and colonic LP (right panel). Each dot in the boxes represents each mouse which were untreated with DSS (n = 3) or fed DSS water for 3 cycles (n = 6). Non-parametric analysis (Mann-Whitney test) was used to evaluate significance. * means < 0.05

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