Fig. 3From: miR32-5p promoted vascular smooth muscle cell calcification by upregulating TNFα in the microenvironmentmiR32-5p up-regulated TNFɑ producing in BV2 cells. a The mRNA expression of TNFɑ in BV2 cells after LPS stimulated by qRT-PCR analyzed. b The protein expression of TNFɑ in BV2 cells after LPS stimulated by Dot-ELISA analyzed. c The gray value analysis of Dot-ELISA. Bars represent mean ± S.D. (n ≥ 3). Significant difference marked with one (p < 0.05) or two (p < 0.01) asterisksBack to article page