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Fig. 4 | BMC Immunology

Fig. 4

From: miR32-5p promoted vascular smooth muscle cell calcification by upregulating TNFα in the microenvironment

Fig. 4

Analysis of the target gene of miR32-5p. a The candidate target genes of miR32-5p by bioinformatics analyzed, the three genes in intersection were RSBN1, PIKfyve and ZFC3H1. b The stem-loop of miR32 and the conserved sites in the 3′ UTR of PIKfyve for miR32-5p. c The wild type and mutation PIKfyve3’ UTR of the seed region of miR-32. d The analysis of miR32-5p target PIKfyve by luciferase assays. e The expression of PIKfyve in BV2 cells after transfected with miR32-5p mimics or antagomir by qRT-PCR analysis. f The expression of PIKfyve in BV2 cells after transfected with miR32-5p mimics or antagomir by Dot-ELISA analysis. g The gray value analysis for Dot-ELISA. Bars represent mean ± S.D. (n ≥ 3). Significant difference marked with one (p < 0.05) or two (p < 0.01) asterisks

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