Fig. 3

a. CD5+ and CD5- B-cell subpopulations pre-immunization (day 0) and post-immunization (day 7) with 13-valent pneumococcal conjugate vaccine in patients with severe chronic kidney disease. Peripheral blood mononuclear cells were stained for CD19 and CD5 and analyzed by flow cytometry. The geometric means are displayed. CD5+ and CD5- B-cell subpopulations are compared between pneumococcal vaccine naïve (day 0, n = 14; day 7, n = 15) and previously immunized with 23-valent pneumococcal polysaccharide vaccine (PPV23) (day 0, n = 19; day 7, n = 24) patients. Statistical significance *p < 0.05 (Mann-Whiney U test for day 0 CD5+, CD5- and day 7 CD5- B cells, Student’s t-test for day 7 CD5+ B cells). b. Absolute numbers of CD5+ and CD5- B-cell subpopulations pre-immunization (day 0) and post-immunization (day 7) with 13-valent pneumococcal conjugate vaccine in patients with severe chronic kidney disease. Absolute numbers of B-cell subpopulations were determined by multiplying the proportions of B-cell subpopulations by the absolute number of lymphocytes. The geometric means are displayed. Comparison between pneumococcal vaccine naïve (day 0, n = 14; day 7, n = 15) and previously immunized with 23-valent pneumococcal polysaccharide vaccine (PPV23) (day 0, n = 19; day 7, n = 24) patients is displayed. Statistical significance *p < 0.05 (Student’s t-test for day 0 CD5+ B cells, Mann-Whitney U test for day 7 CD5+ B cells)