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Fig. 3 | BMC Immunology

Fig. 3

From: Amentoflavone ameliorates cold stress-induced inflammation in lung by suppression of C3/BCR/NF-κB pathways

Fig. 3

AF decreased the level of C3 in lung tissue of cold stress-treated rats (n = 6 per group). a Representative C3 staining in pneumonocytes by immunofluorescence and the ratio of C3-positive cells in each group. Sections are 5 μm thick and photomicrographs are taken at 400×. C3-positive cells were labeled by green fluorescence, DAPI restrained cell nuclei were labeled with blue fluorescence. b AF inhibited the level of C3 fragment deposition on hRBCs in cold stress-treated mice. Representative flow cytometry plots of C3 fragment deposition on hRBCs. Bar graphs showed mean values of three experiments. c AF reduced protein expression levels of C3 by western blotting. d AF reduced the gene expression of C3 in cold stress-treated rat by real-time PCR. e AF increased the level of C3 in serum of cold stress-treated rat. f The refined docking of C3-AF complex. The blue compound represents AF. The data are presented as the mean ± SD. ** P < 0.01, * P < 0.05 vs. cold group; ## P < 0.01 vs. control group

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