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Fig. 1 | BMC Immunology

Fig. 1

From: A combination of the activation marker CD86 and the immune checkpoint marker B and T lymphocyte attenuator (BTLA) indicates a putative permissive activation state of B cell subtypes in healthy blood donors independent of age and sex

Fig. 1

Gating strategy for the different B lymphocyte subtypes from whole blood. The lymphocyte gate was based on FSC and SSC characteristics, followed by a gating of lymphocyte singlets by FSC-H and FSC-A. Plasmablasts (PB) were defined as being CD19 + CD24-CD38++CD27 + CD20-, pre-plasmablasts (pre- PB) as CD19 + CD24-CD38++CD20+ and transitional B cells (TB) as CD19 + CD24++CD38++. The naïve B cells were defined as CD19 + CD27-IgD+ excluding the TB. The switched memory B cells (SM) were defined as CD19 + CD27 + IgD-, the non-switched memory B cells (NSM) were defined as CD19 + CD27 + IgD+, and the double negative memory B cells (DNM) were defined as CD19 + CD27-IgD-. All B cell subtypes were analyzed for CD86 and BTLA expressions, expressed as mean fluorescence intensity (MFI)

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BMC Immunology

ISSN: 1471-2172

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