Fig. 2

a CD86 expression plotted as mean fluorescence intensity (MFI) with boxplots, for B cell subtypes: naïve, pre-plasmablasts (pre-PB), plasmablasts (PB), transitional B cells (TB), switched memory cells (SM), non-switched memory cells (NSM), and double negative memory cells (DNM). #PB CD86 expression was significantly higher compared to all other cell subtypes (P < 0.0001). ¤CD86 expression on pre-PB was higher compared to all other celltypes (p < 0.0001) except PB. ^CD86 expression on SM was higher than on naïve (p < 0.0001), TB (p < 0.0001), NSM (p = 0.002) and DNM (p < 0.001) B cell subpopulations. ~Expression on NSM and DNM were higher than on naïve (p < 0.0001) and TB (p < 0.0001) whereas no significant differences were found between NSM and DNM. All pvalues corrected with Bonferroni-Holm method. Figure is based on results from analyses of samples from 44 blood donors. b CD86 expression with regard to age. Figure is based on results from analyses of samples from 22 blood donors < 41 years, and 22 blood donors > = 41 yrs. c. CD86 expression with regard to sex. Figure is based on results from analyses of samples from 23 women and 21 men. The boxplots shows median, with the upper and lower quartile as box limits and whiskers as min and max if no outliers are present. Outliers are marked with a circle (1,5–3 IQR) or an asterisk (> 3 IQR)