Fig. 4

CPT and LP methods maintain equivalent T cell functionality in isolated PBMCs. a Gating to identify CD4+ and CD8+ T cells (upper) and biaxial plots showing intracellular cytokine production for IL-17A, IFNγ, and IL-4 in CD4+ and CD8+ T cells after stimulation with CEF, PMA-I, or unstimulated control (unstim). One representative PBMC sample is shown. b, c Quantification of indicated intracellular cytokine production in CD4+ and CD8+ T cells from PBMCs isolated by CPT or LP methods following stimulation with b CEF or c PMA-I. All data generated by flow cytometry; values shown are normalized to unstimulated control (value subtracted by unstimulated control), horizontal lines indicate mean +/− SEM. Donors 1,2, 3 are depicted by red, black, and blue dots respectively. Three dots are shown per donor which represent single samples processed by three different operators