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Table 1 Statistical analysis shows choice of CPT or LP method is not the major contributor to variance for all parameters measured. Anova variance for contribution of donor, method (CPT or LP), and operator on yield and viability, cell population distribution (populations), and stimulated cytokine expression in CD4+ and CD8+ T cells (CEF stimulated, PMA-I stimulated). Sum squares, sum square contributions, F values, and P values are show for each parameter measured (rows)

From: Functional comparison of PBMCs isolated by Cell Preparation Tubes (CPT) vs. Lymphoprep Tubes

Yield and ViabilityPopulationsPopulations
 WilcoxonPValueSum SquareSum Square ContributionANOVAFValueANOVAPValue WilcoxonPValueSum SquareSum Square ContributionANOVAFValueANOVAPValue WilcoxonPValueSum SquareSum Square ContributionANOVAFValueANOVAPValue
Cells/mL Pre FreezeMonocytesMemory CD4+
Method9.77E-021.11E+ 1221.4%6.492.56E-02Method5.73E-019.34E-010.4%0.345.70E-01Method3.01E-018.93E-020.0%0.048.45E-01
Donor 3.65E+ 1270.8%10.732.13E-03Donor 2.43E+ 0294.0%44.572.79E-06Donor 9.39E+ 0298.3%209.594.65E-10
Operator 4.01E+ 117.8%1.183.41E-01Operator 1.45E+ 015.6%2.661.10E-01Operator 1.65E+ 011.7%3.685.66E-02
Cells/mL Post FreezeLymphocytesNaïve CD4+
Method1.29E-012.42E+ 119.7%3.399.06E-02Method4.26E-011.60E+ 000.4%0.604.53E-01Method4.26E-011.02E+ 000.1%0.614.50E-01
Donor 2.15E+ 1286.3%15.075.33E-04Donor 3.40E+ 0295.0%63.764.05E-07Donor 1.19E+ 0399.3%355.422.09E-11
Operator 1.01E+ 114.0%0.705.14E-01Operator 1.61E+ 014.5%3.038.62E-02Operator 6.94E+ 000.6%2.071.68E-01
% RecoveryNK CellsMemory CD4-
Method1.29E-017.26E+ 0112.0%2.471.42E-01Method8.23E-018.48E-010.5%0.315.91E-01Method2.50E-011.61E+ 000.6%1.392.62E-01
Donor 2.93E+ 0248.4%4.972.68E-02Donor 1.69E+ 0295.5%30.392.01E-05Donor 2.74E+ 0298.2%118.241.27E-08
Operator 2.40E+ 0239.6%4.074.48E-02Operator 7.15E+ 004.0%1.293.12E-01Operator 3.42E+ 001.2%1.472.68E-01
Viability Pre FreezeT CellsNaïve CD4-
Method1.64E-012.05E+ 0126.6%3.727.79E-02Method9.77E-029.44E+ 005.8%3.081.05E-01Method2.50E-013.40E+ 002.0%2.351.51E-01
Donor 9.17E+ 0011.9%0.834.59E-01Donor 1.22E+ 0274.5%19.871.55E-04Donor 1.51E+ 0290.4%52.131.21E-06
Operator 4.73E+ 0161.5%4.293.92E-02Operator 3.22E+ 0119.7%5.262.29E-02Operator 1.25E+ 017.5%4.343.83E-02
Viability Post FreezeB Cells      
Method9.77E-021.79E+ 0158.7%4.106.56E-02Method3.59E-011.23E-010.9%0.276.12E-01      
Donor 8.18E+ 0026.8%0.944.18E-01Donor 1.24E+ 0192.7%13.767.84E-04      
Operator 4.41E+ 0014.5%0.516.15E-01Operator 8.63E-016.4%0.954.13E-01      
CEF StimulatedPMA-I StimulatedUnstimulated
 WilcoxonPValueSum SquareSum Square ContributionANOVAFValueANOVAPValue WilcoxonPValueSum SquareSum Square ContributionANOVAFValueANOVAPValue WilcoxonPValueSum SquareSum Square ContributionANOVAFValueANOVAPValue
CD4 + IFNg+CD4 + IFNg+CD4 + IFNg+
Method8.20E-012.80E-060.4%0.196.74E-01Method4.96E-017.70E-010.7%0.524.87E-01Method9.10E-012.94E-070.0%0.038.69E-01
Donor 7.74E-0497.9%25.654.64E-05Donor 1.08E+ 0299.0%36.098.39E-06Donor 6.38E-0499.1%30.971.83E-05
Operator 1.40E-051.8%0.466.40E-01Operator 3.10E-010.3%0.109.02E-01Operator 5.31E-060.8%0.267.77E-01
CD4 + IL-17+CD4 + IL-17+CD4 + IL-17+
Method6.73E-015.00E-090.1%0.019.10E-01Method4.96E-012.35E-022.1%0.385.48E-01Method5.79E-024.36E-0727.8%3.209.87E-02
Donor 3.30E-0681.2%4.433.63E-02Donor 1.03E+ 0092.8%8.365.31E-03Donor 7.60E-0748.4%2.801.01E-01
Operator 7.60E-0718.7%1.023.90E-01Operator 5.57E-025.0%0.456.46E-01Operator 3.73E-0723.8%1.372.90E-01
CD4 + IL-4+CD4 + IL-4+CD4 + IL-4+
Method1.64E-016.47E-0453.1%1.712.16E-01Method2.50E-014.74E-018.3%1.832.01E-01Method4.26E-012.55E-048.8%0.445.18E-01
Donor 3.53E-0428.9%0.476.38E-01Donor 4.92E+ 0086.3%9.503.36E-03Donor 1.38E-0347.7%1.213.33E-01
Operator 2.20E-0418.0%0.297.53E-01Operator 3.08E-015.4%0.595.68E-01Operator 1.27E-0343.6%1.103.63E-01
CD8 + IFNg+CD8 + IFNg+CD4-IFNg+
Method3.74E-011.49E-030.2%0.117.45E-01Method3.59E-011.61E+ 010.4%1.013.35E-01Method6.78E-011.20E-059.5%0.783.93E-01
Donor 9.01E-0191.6%33.381.25E-05Donor 4.09E+ 0399.3%128.587.85E-09Donor 8.00E-066.3%0.267.74E-01
Operator 8.09E-028.2%3.008.80E-02Operator 1.39E+ 010.3%0.446.55E-01Operator 1.07E-0484.2%3.496.38E-02
CD8 + IL-17+CD8 + IL-17+CD4-IL-17+
Method1.98E-016.81E-0720.2%3.658.04E-02Method3.01E-011.77E-033.8%1.073.20E-01Method4.19E-011.28E-0617.4%1.772.08E-01
Donor 2.43E-0671.9%6.521.21E-02Donor 4.06E-0285.9%12.281.25E-03Donor 5.61E-0676.2%3.885.03E-02
Operator 2.68E-077.9%0.725.08E-01Operator 4.91E-0310.4%1.492.65E-01Operator 4.74E-076.4%0.337.27E-01
CD8 + IL-4+CD8 + IL-4+CD4-IL-4+
Method7.34E-011.02E-049.9%0.315.87E-01Method3.59E-016.41E-030.0%0.077.92E-01Method1.00E+ 001.72E-050.4%0.028.85E-01
Donor 4.89E-0447.5%0.744.96E-01Donor 1.62E+ 0198.6%91.815.33E-08Donor 2.95E-0373.3%1.861.99E-01
Operator 4.37E-0442.5%0.675.32E-01Operator 2.16E-011.3%1.233.27E-01Operator 1.05E-0326.2%0.665.33E-01